Figure 4.

AA Stimulates Rapid PDGFRα and PLK4 Phosphorylation

Rat primary adventitial fibroblasts were cultured, starved, and pretreated with vehicle (DMSO) or an inhibitor, as described in Figure 1. The arrow points to phospho-protein bands. (A) Representative Western blots showing the time course of AA-induced phosphorylation of kinases. Western blots detect a phospho-protein (p-) and its respective total protein (T-). (B) Quantification: mean ± SEM, n ≥3 experiments. One-way ANOVA/Bonferroni test: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 compared with the 0-min control; p-S6K was included as a downstream effector of the AKT pathway. PDGFR = platelet-derived growth factor receptor; other abbreviations as in Figure 1.