Figure 5.

PDGFR Inhibition Blocks the Phosphorylation of Downstream Kinases

Rat primary adventitial fibroblasts were cultured, starved, and pretreated with vehicle (DMSO) or PDGFR-selective inhibitor crenolanib (1 μmol/l, 30 min). The arrow points to the bands of phospho-PDGFRα that were sensitive to both AA stimulation and inhibitor pretreatment. (A) Representative Western blots showing blockade of AA-induced kinase activation by the PDGFR-selective inhibitor crenolanib. p- = phospho-protein; T- = total protein. (B) Quantification: mean ± SEM, n ≥3 experiments. One-way ANOVA/Bonferroni test: #p < 0.05; ##p < 0.01; ###p < 0.001. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 compared with the vehicle control without AA stimulation (the first bar in each plot). Abbreviations as in Figures 1 and 4.