Fig. 1.

Different effect of homocysteine on Akt activity in HUVEC and SH-SY5Y cell lines. Immunoblotting for Akt and p-Akt using lysates from A HUVEC, B SH-SY5Y cells having been treated with 3 mM Hcy for the indicated time periods. Blots were stripped and reprobed with anti-β-actin antibody to normalize for differences in protein loading. Bar graphs on the right panel of the figure present the mean values ± SEM of three independent experiments relative to control where “a”, represents significant difference (p < 0.05) relative to control (one-way ANOVA followed by Dunnett’s multiple comparison test). C Immunoblotting for Akt and p-Akt using lysate from HUVEC cells following treatment of HUVEC cells for 24 h with 3 mM Hcy, with or without a 2 h pre-treatment with 25 μM DFO. Blots were stripped and reprobed with anti-β-actin antibody to normalize for differences in protein loading. Significance of differences between samples was evaluated by one-way ANOVA followed by Tukey’s multiple comparison test. Bar graphs on the right panel of the figure present the mean ± SEM of three independent experiments relative to control where “a” represents statistical significance of p < 0.05 as compared to control