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. 2020 Aug 13;60(3):1619–1631. doi: 10.1007/s00394-020-02360-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Homocysteine-induced increase in ferritin L and H levels in HUVEC cells. Immunoblotting for A ferritin L and B ferritin H using lysate from HUVEC cells having been treated with 3 mM Hcy for the indicated time periods. Immunoblotting for C ferritin L and D ferritin H using lysate from SH-SY5Y cells having been treated with 3 mM Hcy for the indicated time periods. Blots were stripped and reprobed with anti-β-actin antibody to normalize for differences in protein loading. Graphs on the right panel of the figure present the mean ± SEM of three independent experiments relative to control where "a" represents statistical significance at p < 0.05 as compared to control (one-way ANOVA followed by Dunnett’s multiple comparison test)