Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 13;60(3):1619–1631. doi: 10.1007/s00394-020-02360-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Role of FOXO3a in homocysteine-induced ferritin upregulation. A Immunoblotting for p-FOXO3a using lysate from HUVEC cells having been treated with 3 mM Hcy for the indicated time periods. The numbers on top of the immunoreactive bands represent a change in protein levels relative to control. B Immunoblotting for ferritin L, ferritin H and FOXO3a using lysate from HUVEC cells transfected with siRNA against FOXO3a. Blots were stripped and reprobed with anti-β-actin antibody to normalize for differences in protein loading. Bar graphs on the right panel of the figure present the mean ± SEM of three independent experiments relative to control where "a" represents statistical significance at p < 0.05 as compared to control (paired t test). C Ferritin L and ferritin H mRNA levels in HUVEC cell line transfected with siRNA against FOXO3a. mRNA levels were measured by qPCR. Data are shown as means ± SD of three independent experiments carried out in duplicate ("a" represents significance at p < 0.05, as compared with control siRNA transfected cells ( paired t test)