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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: FEBS Lett. 2020 Nov 5;595(6):799–810. doi: 10.1002/1873-3468.13957

Figure 3: Subcellular localization of the rAbcc6 fusion proteins in HEK293 cells.

Figure 3:

A: representative image of the subcellular localization of the wild type, unmodified rAbcc6 protein overexpressed in HEK293 cells by immunofluorescence with an 40X objective and 3X optical zoom. B-H: representative images of the subcellular localization of the fluorescent fusion proteins by live cell confocal microscopy Panels B-E show the localization of the green fluorescent rAbcc6 fusion proteins with an 60X objective and 3X optical zoom (B: rAbcc6-mNG_ICL1; C: rAbcc6-mNG_ECL3; D: rAbcc6-mNG_bNBD1; E: rAbcc6-mNG_aNBD1. Panel F: rAbcc6E1426Q-mNG_aNBD1). Panels G and H show representative images of the red fluorescent rAbcc6 fusion proteins (G: His10-rAbcc6_mSc_aNBD1; H: FLAG-C3-rAbcc6-mSc_aNBD1). All scale bars represent 30 µm.