Table 1.
USER PCR primers and templates used in the generation of the rAbcc6 fusion constructs.
| construct | template | forward primer | reverse primer |
|---|---|---|---|
| His10-rAbcc6 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ATGGTGGUGGTGATGATGATGCATGCCAACTTTTTTGTACAA |
| pDonr223_rAbcc6 | ACCACCAUCATCATCATGAGTCCTGCGCAGGCCTG | AGCACACAUTCTCCACCACAGAGGTATTC | |
| pDonr223_rAbcc6 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| His10-rAbcc6-mSc_aNBD1 | pDonr223_His10_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ACAGCAUGTGCTTCTCCTTCTCCTT |
| pmSc-I C1 | ATGCTGUGGACATGGTGAGCAAGGGCGAG | ACTGGTGGCUGCTCTGGGCTTGTACAGCTCGTCCATGC | |
| pDonr223_His10_rAbcc6 | AGCCACCAGUGATGACCTTGGAGGCTTT | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| Flag-C3-rAbcc6-mSc-1_aNBD1 | pDonr223_rAbcc6 clone 6.3 | AGGCGCAGCGGUCGGGCTGAACGGGGGGTT | AGCTTGUCGTCATCGTCTTTGTAGTCCATGCCAACTTTTTTGTA CAAAGTTGGCA |
| pDonr223_rAbcc6 clone 6.3 | ACAAGCUGGAGGTGCTGTTCCAGGGTCCCAACGG AGAGCACTCAATGGCCACGC | AACTCCUGCAGGGAAGCTGGCCACATCA | |
| pDonr223_His10_rAbcc6_mSc aNBD1 | AGGAGTUCACACCCCAGTAGGGGAGC | ACCGCTGCGCCUTATCCGGTAACTATCGTC | |
| rAbcc6-mNG_ICL1 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ACCATGUCCACGCAGCCATGGCGATGGAT |
| pDonr223_rAbcc6 | AGCCCAGAUGCTACCTCCGGATGTCCC | AGCACACAUTCTCCACCACAGAGGTATTC | |
| pDonr223_rAbcc6 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| L40C-Crispr EFS.mNeon | ACATGGUGTCCAAGGGCGAAGAGGACAACA | ATCTGGGCUTGTACAGCTCGTCCATGCCCATCA | |
| rAbcc6-mNG_ECL3 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ACACCAUGGAGGCCTGCTGGACAGT |
| pDonr223_rAbcc6 | AGGGAGCUTCCGCCAGGAGCCCCTCC | AGCACACAUTCTCCACCACAGAGGTATTC | |
| pDonr223_rAbcc6 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| L40C-Crispr EFS.mNeon | ATGGTGUCCAAGGGCGAAGAGGACA | AGCTCCCUGCCTTGTACAGCTCGTCCATG | |
| rAbcc6-mNG_L0 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ACCATGUGCCCCGGCAGCTCACTGA |
| pDonr223_rAbcc6 | AGGGCACAGUGGTATGGGGACCCCCGAGACAGA | AGCACACAUTCTCCACCACAGAGGTATTC | |
| pDonr223_rAbcc6 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| L40C-Crispr EFS.mNeon | ACATGGUGTCCAAGGGCGAAGAGGA | ACTGTGCCCUTTCTTGTACAGCTCGTCCATG | |
| rAbcc6-mNG_ECL4 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ACCATCUCGAGGTCGCCCATGAACT |
| pDonr223_rAbcc6 | ACAAGTCCUCGGCTTGGACGGGCTGGC | AGCACACAUTCTCCACCACAGAGGTATTC | |
| pDonr223_rAbcc6 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| L40C-Crispr EFS.mNeon | AGATGGUGTCCAAGGGCGAAGAGGACAAC | AGGACTTGUACAGCTCGTCCATGCCCATCA | |
| rAbcc6-mNG_ECL6 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ACCATCGCAUCCATGGCGTTGTCCTCTG |
| pDonr223_rAbcc6 | AGAAGGCGUTTGTGACGCTCACGGTGCTC | AGCACACAUTCTCCACCACAGAGGTATTC | |
| pDonr223_rAbcc6 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| L40C-Crispr EFS.mNeon | ATGCGATGGUGTCCAAGGGCGAAGAGGA | ACGCCTTCUCCTTGTACAGCTCGTCCATG | |
| rAbcc6-mNG_bNBD1 | pDonr223_rAbcc6 | AGACCCUCCAGATGCTCCTCGAAGGATCGA | ATGTCCACACUCAAGACCATGCCATTGGGGTCTA |
| L40C-Crispr EFS.mNeon | AGTGTGGACAUGGTGTCCAAGGGCGAAGA | AGGGTCUGGGCTTGTACAGCTCGTCCATG | |
| rAbcc6-mNG_aNBD1 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | ACAGCAUGTGCTTCTCCTTCTCCTT |
| pDonr223_rAbcc6 | AGCCACCAGUGATGACCTTGGAGGCTTT | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| L40C-Crispr EFS.mNeon | ATGCTGUGGACATGGTGTCCAAGGGCGAA | ACTGGTGGCUGCTCTGGGCTTGTACAGCTCGTCCATG | |
| rAbcc6E1426Q-mNG_aNBD1 | pDonr223_rAbcc6_mNG_aNBD1 | AGGCGCAGCGGUCGGGCTGAACGGGGGGTT | AGCACACAUTCTCCACCACAGAGGTATTC |
| pDonr223_rAbcc6_mNG_aNBD1 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | AGTGGCTUGATCCAGGATGAGGATCTGGGTTTTCCGGAGAAG | |
| pDonr223_rAbcc6_mNG_aNBD1 | AAGCCACUGCCTCCGTGGACCCAGGGA | ACCGCTGCGCCUTATCCGGTAACTATCGTC | |
| rAbcc6-mNG_ECL7 | pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | AGCACACAUTCTCCACCACAGAGGTATTC |
| pDonr223_rAbcc6 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | ACCATCCCAUCCACGACCGGGTCGTCGG | |
| pDonr223_rAbcc6 | AGAAGCAGAUGCATTCAGCCCTGCGTGGC | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| L40C-Crispr EFS.mNeon | ATGGGATGGUGTCCAAGGGCGAAGAGGACAACA | ATCTGCTTCUTGTACAGCTCGTCCATGCCCATCA | |
| rAbcc6-TGLAT-mNG | pDonr223_rAbcc6_TGLAT_TEV_GFP_His10 | ATGTGTGCUTCAGGCAGGAGCTGGATCTG | ACCATTGUAGCAAGCCCGGTGGCTAG |
| pDonr223_rAbcc6_TGLAT_TEV_GFP_His10 | ACAAGACUAGTCGTGGTTCTCATCATC | AAAGAACAUGTGAGCAAAAGGCCAGCAA | |
| pDonr223_rAbcc6 | ATGTTCTTUCCTGCGTTATCCCCTG | AGCACACAUTCTCCACCACAGAGGTATTC | |
| L40C-Crispr EFS.mNeon | ACAATGGUGTCCAAGGGCGAAGAGGACAAC | AGTCTTGUACAGCTCGTCCATGCCCATCA |