Figure 1.

Genetic Design A: Insertion of loxP in mouse Apoa1 allele at Intron 1 and Intron 3 using CRISPR/Cas9 technique. The sequence indicated at genome alleles are complementally sequence for sgRNA and PAM sequences. Donor oligonucleotides including loxP sequence were described, respectively. The open arrow indicated the cleavage sites by cas9. B: The genotyping primers positions of Apoa1^fl/fl mice and cre transgenic mice. Solid blue boxes indicated the loxP insertion sites. The primer sets for each allele’s genotyping were indicated in arrows.