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. 2021 Mar 10;12:626621. doi: 10.3389/fphar.2021.626621

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Copyright © 2021 Zhao, Du, Zhao, Ji, Huang, Pang, Guo and Yin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of catalpol on osteogenic differentiation and calcification of MC3T3-E1 cells treated by high glucose. (A) Cell differentiation was measured by ALP staining. (B) Cell calcification was measured by Alizarin Red staining. (C) Catalpol reversed cell calcification according to microplate reader of Alizarin Red staining measured at 562 nm. (D) Catalpol increased cell ALP activity treated with high glucose. The data were expressed as the mean ± SD. **p < 0.01 vs. control and ^## p < 0.01, and ^# p < 0.05 vs. model.