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. 2021 Mar 24;12(4):310. doi: 10.1038/s41419-021-03513-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Immunoblotting (IB) of flag-immunoprecipates (IP) and whole cell lysis (WCL) derived from HEK293T cells that were transfected with HA-SARS-CoV-2 S and indicated NEDD4 family members. B A schematic diagram to show the PPxY motif in the SARS-CoV-2 S protein. C IB of flag-immunoprecipates and WCL derived from HEK293T cells that were transfected with NEDD4 and SARS-CoV-2 S WT or mutants. D IB of Ni-NTA pulldown and WCL derived from HEK293T cells that were transfected with SARS-CoV-2 S and NEDD4 WT (or EV as a negative control).