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. 2021 Mar 24;12(4):310. doi: 10.1038/s41419-021-03513-1

Fig. 5. Gain-of-Function mutants in NEDD4, but not SMURF1 and WWP1, display elevated interaction with, but comparable ubiquitination of the SARS-CoV-2 S protein.

Fig. 5

A, C IB of flag-immunoprecipates and WCL derived from HEK293T cells that were transfected with HA-SARS-CoV-2 S and NEDD4/WWP1 WT and mutants. B, D IB of Ni-NTA pulldown and WCL derived from HEK293T cells that were transfected with SARS-CoV-2 S and NEDD4/WWP1 WT and mutants. E IB of flag-immunoprecipates and WCL derived from HEK293T cells that were transfected with HA-SARS-CoV-2 S and WT and mutants for Smurf1 or WWP1. F IB of Ni-NTA pulldown and WCL derived from HEK293T cells that were transfected with SARS-CoV-2 S and WT and mutants for Smurf1 or WWP1. G Binding and ubiquitination activity in WWP1/NEDD4/SMURF1 mutants vs. wild-type (WT).