Table 2.
Real-Time PCR primer sequences.
| Gene | Accession number | Sequence (5’→3’) | Product size (bp) | |
|---|---|---|---|---|
| WWP1 | NM_007013.4 | Fw | TGTAAATGTTACGCCACAGACT | 105 |
| Rv | GCTTGTTTCAAATCTATCGTTGC | |||
| WWP2 | NM_007014.5 | Fw | GAAAGTGGTGTCCGCAAAGC | 175 |
| Rv | ATGACTCTGTGCCGTGACATT | |||
| NEDD4 | NM_006154.4 | Fw | CTGCTACGGACAATTATACCCTA | 129 |
| Rv | CATCCAACAGTTTGCCATGATA | |||
| NEDD4L | NM_001144967.3 | Fw | ACGTAGCGGATGAGAATAGAGAAC | 115 |
| Rv | CTGTGATTAGATGGGTTTACCCTGA | |||
| ITCH | NM_031483.7 | Fw | GGTTCAGTATTTCCGGTTCTGGT | 118 |
| Rv | GGGACTGAAGCTCATTATCTGTTG | |||
| SMURF1 | NM_020429.3 | Fw | CCGCTCCAAGGCTTCAAGG | 125 |
| Rv | ATCCGGTTAAAGCAGGTATGGG | |||
| SMURF2 | NM_022739.4 | Fw | GCAAATGGATCAGGAAGTCGGAAA | 100 |
| Rv | CCGGAGGCCGGAGGA | |||
| HECW1 | NM_015052.5 | Fw | CGAGCAACCACCCCCAGTGT | 136 |
| Rv | CCATGGCTTGGAAATCTGAGAGA | |||
| HECW2 | NM_001348768.2 | Fw | CTACCAGCATAACCGCGACC | 112 |
| Rv | AAAGAATGCCTTGCCCTGGT | |||
| GAPDH | NM_002046 | Fw | AAGGTCGGAGTCAACGGATTT | 100 |
| Rv | TGAAGGGGTCATTGATGGCA | |||
| ACTB | NM_001101 | Fw | ATTGCCGACAGGATGCAGAA | 150 |
| Rv | GCTGATCCACATCTGCTGGAA | |||
| RPLP0 | NM_001002 | Fw | ACCCAGCTCTGGAGAAACT | 198 |
| Rv | AAAAGGAGGTCTTCTCGGG | |||
WWP1 WW domain containing E3 ubiquitin protein ligase 1, WWP2 WW domain containing E3 ubiquitin protein ligase 2, NEDD4 neural precursor cell expressed, developmentally downregulated 4, E3 ubiquitin protein ligase, NEDD4L neural precursor cell expressed, developmentally downregulated 4-like, E3 ubiquitin protein ligase, ITCH HECT-type E3 ubiquitin transferase itchy homolog, SMURF1 SMAD specific E3 ubiquitin protein ligase 1, SMURF2 SMAD specific E3 ubiquitin protein ligase 2, HECW1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1, HECW2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2, GAPDH glyceraldehyde-3-phosphate dehydrogenase, ACTB β-actin, RPLP0 ribosomal protein, large, P0, Fw forward, Rev reverse. PCR polymerase chain reaction.