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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Metab Eng. 2021 Jan 19;64:41–51. doi: 10.1016/j.ymben.2021.01.004

Figure 4.

Figure 4

In vivo production of hydroxycineole by E. coli DH1 strains with engineered enzyme fusions. (A) Metabolic pathway of hydroxycineole production from glucose using enzyme fusions of CS and P450cin, CS-(GSG)n-P450cin (n = 1–5). (B) OD600 of production strains after 48 hours. (C) Production of 1,8-cineole and hydroxycineole with or without the solvent overlay. Hydroxycineole concentrations are reported based on the equivalent concentration of 1,8-cineole. Error bars indicate one standard deviation of triplicates.