Table 1.
Strains and plasmids used in this study
| Strains | Description | Reference |
|---|---|---|
| CS | E. coli BL21 (DE3) with pSKB3-CS | This study |
| CinA | E. coli BL21 (DE3) with pSKB3-CinA | This study |
| CinC | E. coli BL21 (DE3) with pSKB3-CinC | This study |
| G1 | E. coli BL21 (DE3) with pSKB3-CS-(GSG)1-CinA | This study |
| G2 | E. coli BL21 (DE3) with pSKB3-CS-(GSG)2-CinA | This study |
| G3 | E. coli BL21 (DE3) with pSKB3-CS-(GSG)3-CinA | This study |
| G4 | E. coli BL21 (DE3) with pSKB3-CS-(GSG)4-CinA | This study |
| G5 | E. coli BL21 (DE3) with pSKB3-CS-(GSG)5-CinA | This study |
| Fpr | E. coli BL21 (DE3) with pSKB3-Fpr | This study |
| 2pCin_Non-fusion | E. coli DH1 with JBEI-3122 + pTrc99a-trGPPS-CS-RBS-CinA-CinC | This study |
| CS-(GSG)1-P450cin | E. coli DH1 with JBEI-3122 + pTrc99a-trGPPS-CS-(GSG)1-CinA-CinC | This study |
| CS-(GSG)2-P450cin | E. coli DH1 with JBEI-3122 + pTrc99a-trGPPS-CS-(GSG)2-CinA-CinC | This study |
| CS-(GSG)3-P450cin | E. coli DH1 with JBEI-3122 + pTrc99a-trGPPS-CS-(GSG)3-CinA-CinC | This study |
| CS-(GSG)4-P450cin | E. coli DH1 with JBEI-3122 + pTrc99a-trGPPS-CS-(GSG)4-CinA-CinC | This study |
| CS-(GSG)5-P450cin | E. coli DH1 with JBEI-3122 + pTrc99a-trGPPS-CS-(GSG)5-CinA-CinC | This study |
| P450cin-(GSG)3- CS | E. coli DH1 with JBEI-3122 + pTrc99a-trGPPS-CinA-(GSG)3-CS-CinC | This study |
| 2pEiz | E. coli DH1 with JBEI-2704 + JBEI-15862 | This study |
| 2pEiz_Non-fusion | E. coli DH1 with JBEI-2704 + pTrc99a-EizS-RBS-CYP170A1-CinC | This study |
| EizS-(GSG)1-CYP170A1 | E. coli DH1 with JBEI-2704 + pTrc99a-EizS-(GSG)1-CYP170A1-CinC | This study |
| EizS-(GSG)2-CYP170A1 | E. coli DH1 with JBEI-2704 + pTrc99a-EizS-(GSG)2-CYP170A1-CinC | This study |
| EizS-(GSG)3-CYP170A1 | E. coli DH1 with JBEI-2704 + pTrc99a-EizS-(GSG)3-CYP170A1-CinC | This study |
| EizS-(GSG)4-CYP170A1 | E. coli DH1 with JBEI-2704 + pTrc99a-EizS-(GSG)4-CYP170A1-CinC | This study |
| EizS-(GSG)5-CYP170A1 | E. coli DH1 with JBEI-2704 + pTrc99a-EizS-(GSG)5-CYP170A1-CinC | This study |
| Plasmids | Description | Reference |
| pSKB3 | Modified pET-28a | (Kang et al., 2016) |
| JBEI-3122 | pBbA5c-MTSA-T1-MBI | (Alonso-Gutierrez et al., 2013) |
| JBEI-15065 | pTrc99a-GPPS-CSStr | (Mendez-Perez et al., 2017) |
| JBEI-2704 | pBbA5c-MevT-T1-MBIS | (Redding-Johanson et al., 2011) |
| JBEI-15862 | pTrc99a-coEizS | (Liu et al., 2018) |
| JPUB_016968 | pSKB3-CS | This study |
| JPUB_016970 | pSKB3-CinA | This study |
| JPUB_016972 | pSKB3-CinC | This study |
| JPUB_016974 | pSKB3-CS-G1-CinA | This study |
| JPUB_016976 | pSKB3-CS-G2-CinA | This study |
| JPUB_016978 | pSKB3-CS-G3-CinA | This study |
| JPUB_016980 | pSKB3-CS-G4-CinA | This study |
| JPUB_016982 | pSKB3-CS-G5-CinA | This study |
| JPUB_016984 | pSKB3-Fpr | This study |
| JPUB_016986 | pTrc99a-trGPPS-CS-RBS-CinA-CinC | This study |
| JPUB_016988 | pTrc99a-trGPPS-CS-(GSG)1-CinA-CinC | This study |
| JPUB_016990 | pTrc99a-trGPPS-CS-(GSG)2-CinA-CinC | This study |
| JPUB_016992 | pTrc99a-trGPPS-CS-(GSG)3-CinA-CinC | This study |
| JPUB_016994 | pTrc99a-trGPPS-CS-(GSG)4-CinA-CinC | This study |
| JPUB_016996 | pTrc99a-trGPPS-CS-(GSG)5-CinA-CinC | This study |
| JPUB_016998 | pTrc99a-trGPPS-CinA-(GSG)3-CS-CinC | This study |
| JPUB_017000 | pTrc99a-EizS-RBS-CYP170A1-CinC | This study |
| JPUB_017002 | pTrc99a-EizS-(GSG)1-CYP170A1-CinC | This study |
| JPUB_017003 | pTrc99a-EizS-(GSG)2-CYP170A1-CinC | This study |
| JPUB_017005 | pTrc99a-EizS-(GSG)3-CYP170A1-CinC | This study |
| JPUB_017007 | pTrc99a-EizS-(GSG)4-CYP170A1-CinC | This study |
| JPUB_017009 | pTrc99a-EizS-(GSG)5-CYP170A1-CinC | This study |