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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Immunol. 2021 Mar 1;206(7):1540–1548. doi: 10.4049/jimmunol.1900656

Figure 4: Sod1 is induced by Nrf2 activation but may not be involved in Nrf2-mediated IL-17A and IL-22 responses in Th17 cells:

Figure 4:

A) C57BL/6 (WT) and Nrf2−/− naïve CD4+ T cells were polarized to Th17 cells in presence of CDDO-Im or vehicle control. Differentiated Th17 cells were harvested on day 4 and processed for qPCR on Sod1, Sod2, Sod3 and Cybb.

B) Naïve CD4+ T cells from C57BL/6 mice were polarized to Th17 cells in presence of TEMPOL or vehicle control. After 4 days of culture, cells were stimulated with PMA+Ionomycin. CD4+ T cells (gated on CD3+CD4+) producing IL-17A and IL-22 were analyzed by flow cytometer. Data show the percentage of CD4+ T cells producing IL-17A and IL-22 in response to TEMPOL or control vehicle (DMSO) stimulation.

Figure 4B was generated from 2 independent experiments. Each symbol indicates experiments from a separate animal. Data presented as mean ± SEM on relevant graphs. *P ≤ 0.05; **P ≤ 0.01 (Mann-Whitney test, Two-tailed and One-Way ANOVA).