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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Immunol. 2021 Mar 1;206(7):1540–1548. doi: 10.4049/jimmunol.1900656

Figure 7: Nrf2 binds to ARE of mouse Ahr:

Figure 7:

A) Naïve CD4+ T cells from AhRfl/fl and AhRCD4 mice were polarized to Th17 cells in presence of CDDO-Im or vehicle control for 4 days. Cells were harvested for qPCR analysis on Nqo1 expression.

B) Schematic diagram shows the putative ARE binding motifs in mouse Ahr gene.

C) Naïve CD4+ T cells isolated from the spleens and lymph nodes of C57BL/6 mice were cultured under the Th17 differentiation condition for 4 days. Cells were harvested and processed for CHIP with anti-Nrf2 antibody. The Nrf2-binding DNA was utilized for qPCR analysis to examine the interaction between the ARE motif of Ahr gene and Nrf2.

Data presented as mean ± SEM on relevant graphs. **P ≤ 0.01; ****P ≤ 0.0001 (Paired student t test and One-way ANOVA).