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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Dev Biol. 2021 Jan 28;473:33–49. doi: 10.1016/j.ydbio.2021.01.012

Figure 3: YAP expression during postnatal VF epithelial development.

Figure 3:

(A-D) Transverse sections of mouse VF at P0. IF analysis of K5 (red) (A), total YAP (green) (B), p-YAP (red) (C) and double staining of YAP and p-YAP (yellow) (D). YAP is expressed mainly in the cytoplasm of K5 positive epithelial cells. (E-H) Transverse sections of mouse VF at P7. IF analysis of K5 (red) (E), total YAP (green) (F), p-YAP (red) (G) and double staining of YAP and p-YAP (yellow) (H). YAP continues to be expressed predominantly in the cytoplasm at this stage. (I-L) Transverse sections of mouse VF at P14. IF analysis of K5 (red) (I), total YAP (green) (J), p-YAP (red) (K) and double staining of YAP and p-YAP (yellow) (L). At P14, a distinct cyto-nucleoplasmic shift is noted compared to the previous stage. YAP shifts from the cytoplasm to nucleus in some VF epithelial cells throughout the VF epithelium. White solid arrows in the panels of J, L denote nuclear YAP positive cells. These regions are magnified in the upper right corners of the same panels. (M-P) Transverse sections of mouse VF at P28. IF analysis of K5 (red) (M), total YAP (green) (N), p-YAP (red) (O) and double staining of YAP and p-YAP (yellow) (P). Similar to P14, at P28, a distinct cyto-nucleoplasmic shift is noted. In apical cells, YAP is highly phosphorylated and excluded from the nucleus, while basal cells exhibit prominent nuclear YAP localization. White solid arrows in the panels of N, P denote nuclear YAP positive cells. These regions are magnified in the upper right corners of the same panels. (Q-T) Transverse sections of mouse VF at P42. IF analysis of K5 (red) (Q), total YAP (green) (R), p-YAP (red) (S) and double staining of YAP and p-YAP (yellow) (T). YAP is again primarily localized to the cytoplasm in basal and apical cells. (U-X) Transverse sections of adult mouse VF. IF analysis of K5 (red) (U), total YAP (green) (V), p-YAP (red) (W) and double staining of YAP and p-YAP (yellow) (X). YAP is localized in the cytoplasm. A white dotted line at the panels of B, F, J, N, R, and V denotes basal versus apical cellular compartments. Primary antibodies are color-coded according to their secondary antibodies, and nuclei are counterstained with DAPI. All images are taken at 60X magnification; scale bar is 20 μm. Abbreviations: AC, apical cells; BC, basal cells; EP, epithelium.