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. Author manuscript; available in PMC: 2022 Mar 23.
Published in final edited form as: Circulation. 2021 Jan 19;143(12):1242–1255. doi: 10.1161/CIRCULATIONAHA.120.051889

Figure 4. HF is associated with increased accumulation of Isolevuglandin (IsoLG)-protein adducts.

Figure 4.

(A) Fixed LV tissue sections from non-HF (n=2) and end-stage nonischemic HF patients (n=3) were probed for the presence of IsoLG-protein adducts with D11 scfv recombinant antibody. (B) Frozen LV tissue sections from wt Sham (4wks post-surgery) and TAC (1wk, 2wk and 4wk post-surgery) mice were probed for intracellular ROS by CM-H2DCFDA. (Images representative of n≥3 mice each) (C) CD45+MHCII+CD11b+CD11c+ DCs isolated from the mLNs of wt Sham and TAC mice were identified by FACS. (D-E) DCs mice were then probed for intracellular IsoLG-protein adducts with D11 scfv recombinant antibody and quantified. (F-G) The total number of mature DCs expressing CD80 in wt Sham and TAC mLNs was quantified by FACS. Scale bars: 100μm. Error bars represent mean ± SD. (* p<0.05, *** p<0.001; Mann-Whitney test, one-way ANOVA test for comparison of 3 or more groups).