FIGURE 5.

Na⁺ diffusion in glial networks. (A) Cells in the center of the inferior colliculus can be loaded with the fluorescent Na⁺ indicator SBFI-AM (left). Astrocytes and bona fide oligodendrocytes can be distinguished by sulforhodamine (SR) 101-labeling. (B) Electrical stimulation results in Na⁺ load of a single astrocyte (a₁). Subsequently, neighboring astrocytes (a₃–a₅,a₇; SR⁺) and bona fide oligodendrocytes (a₂,a₆; SR^–), show elevated Na⁺ transients as well. (C,D) Na⁺ diffusion can be elicited by stimulation of both astrocytes (C) and bona fide oligodendrocytes (D). The amplitude of Na⁺ transients depends on the distance from the stimulated cell. Figure modified from Wadle et al. (2018).