TABLE 4.

Summary of turnaround time and cost for each multiplexing strategy.

Multiplexing isolate number	1	3	4	5	7	10
Cost per isolatea	$910	$330	$255	$210	$159	$120
Average sequencing time to obtain at least 50 × depth of genome coverage for each multiplexed Salmonella isolate	<1.0 h	2.9 h	4.0 h	6.0 h	19.5 h	>25.1
Data analysis time	<1.0 h	<2.0 h	<2.0 h	<2.0 h	<3.0 h	<3.0 h
DNA extraction + quality control	3.5 h	3.5 h	3.5 h	4.0 h	4.5 h	4.5 h
Library construction	1.0 h	1.5 h	1.5 h	1.5 h	2.0 h	2.0 h
Total time	6.5 h	9.9 h	11.0 h	13.5 h	29.0 h	34.6 h

^aCost per isolate was calculated based on the market price of DNA extraction and ONT consumables in China in Dec. 2019.