(A-D) IHC of Elnb (green) and Mhc (red) in hearts of (A,B) WT sibling and nls mutant embryos and (C,D) DMSO- and DEAB-treated embryos at 72 hpf. Number of embryos examined per condition: WT n=6, nls n=8, DMSO-treated n=12, DEAB-treated n=9. Views are lateral with arterial pole of the heart up. Arrows indicate the Elnb in WT sibling and DMSO-treated and the lack of Elnb in nls mutant and DEAB-treated at the arterial poles of the hearts. Scale bar – 50 μm.