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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Immunol. 2021 Mar 3;206(7):1505–1514. doi: 10.4049/jimmunol.1901464

Figure 2. Reduced humoral immune response in heterozygous and homozygous mice.

Figure 2.

(A) Concentration of different subtypes of secreted immunoglobulins in plasma of naïve mice over time, determined by Meso scale assay system, n=7-11 per group. (B) Optical density (OD) of antigen-specific antibodies measured in plasma collected 2 weeks after immunization with B. pertussis and chicken-gamma-globulin (CGG) in Alum, n=6-8 per group. (C) Percent of germinal center B cells (GCB) in the spleen 10 and 15 days after immunization with sheep red blood cells (SRBCs), determined by flow cytometry. (D) Number of memory B cells in the spleen 15 days post immunization with SRBCs. (E) Area under curve (AUC) from ELISA of P. falciparum circumsporozoite protein (PfCSP)-specific IgG in the serum 5 days post booster immunization with PfCSP in Alum. (A-E) Columns represent the mean of each group ± SEM, individual mice represented by individual symbols. Data from one experiment. (A-D) One-way, pairwise ANOVA with multiple comparisons assuming equal standard variation. (E) Student’s unpaired T test.