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. 2021 Mar 10;12:610164. doi: 10.3389/fphar.2021.610164

FIGURE 5.

FIGURE 5

Urolithin A antagonizes the TNFα–induced TER decrease and expression changes to claudin-1 and two in HT-29/B6 monolayers (A) 150 and 250 µM urolithin A (UroA) partially prevented the TNFα-caused TER decrease in HT-29/B6 monolayers (***p < 0.001 vs. control; ### p < 0.001 TNFα vs. urolithin A + TNFα; n = 6–12) (B) 150 µM urolithin A impeded TNFα-induced up-regulation of claudin-1 and -2 completely, as shown by representative Western blots and densitometry (*p < 0.05, **p < 0.01, ***p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 TNFα vs. urolithin A + TNFα or urolithin A; n = 5–8) (C) but 150 µM urolithin A did not prevent caspases-3 cleavage caused by TNFα (**p < 0.01 vs. control; n = 5–6).