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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Kidney Int. 2020 Dec 13;99(4):870–884. doi: 10.1016/j.kint.2020.10.038

Figure 3. Inhibition of tubule mitochondria AKT aggravated kidney failure induced by ischemia reperfusion.

Figure 3.

Figure 3.

Figure 3.

(A) The experimental protocol of ischemia reperfusion injury for this series of study. (B, C) Serial measurements showed rise of BUN and Creatinine (Cr) after IRI. Significantly higher BUN and Cr was observed in the male Tam-treated KMDAKT mice on day 45 (*p<0.002, **p<0.001). (D) Renal tissue histology (7 days after IRI) stained with HE to estimate Jablonski scores. OSOM - outer stripe outer medulla. ISOM - inner stripe outer medulla. IM - inner medulla. Tamoxifen injected KSP-CreERT2 mice served as another control for Tamoxifen-KMDAKT mice. The Jablonski scores were summarized in the bar graph. *p < 0.01. (E) Renal fibrosis was analyzed by Masson’s trichrome staining (7 days after IRI). The bar graph compared the extent of renal fibrosis in the two groups. #p < 0.02. (F) Expression of TGFβ and Col1A in kidney (7 days after IRI). The levels of TGFβ and Col1A mRNA were analyzed and expressed as ratios to GAPDH mRNA. # p < 0.01. (G) KIM-1 expression was examined by immunohistochemistry (7 days after IRI). The bar graph summarized the results of KIM-1 staining in these mice. #p < 0.02. (H) Renal apoptosis was analyzed by TUNEL staining (7 days after IRI). Upper panels show representative microscopic images, while lower panels summarized results of quantification of apoptotic cells from the two groups of mice. (I) Activation of caspase 3 and 9 in kidneys (7 days after IRI). Activated caspase 3 and 9 were analyzed by immunohistochemistry. The bar graph summarized the results of staining in these mice. #p < 0.01. (J) Glomerulosclerosis was analyzed by Periodic acid–Schiff (PAS) staining. The PAS results were graded as follows: 0 (no lesions), 1 (lesions in up to 25% of glomeruli), 2 (lesions in 25 to 50% of glomeruli) or 3 (lesions in > 50% of glomeruli). The representative images were shown in the upper panel. At least 50 glomeruli were scored for each mouse, the results were summarized in the graph (lower panel). **p<0.001. NS - not significant. (K) Survival analysis after IRI was compared in the two groups (TAM n=30, Corn oil n=28, all male). 46.7% TAM-treated KMDAKT mice survived by day 45, as compared to 85.7% control mice. Log rank test, p=0.0013.