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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Kidney Int. 2020 Dec 13;99(4):870–884. doi: 10.1016/j.kint.2020.10.038

Figure 4. Augmentation of mitochondria AKT in tubules reversed kidney failure induced by ischemia reperfusion.

Figure 4.

Figure 4.

(A) The experimental protocol of ischemia reperfusion injury for this series of study. (B, C) Acute kidney injury was induced by IR in male KMCAKT mice. BUN and Cr were measured after IRI in the TAM-treated KMCAKT and corn oil-treated KMCAKT mice. Tam-KMCAKT mice showed lower BUN and Cr after day 2 (*p<0.032, **p<0.001). (D) Renal histology (7 days after IRI) was HE stained to estimate Jablonski scores. OSOM - outer stripe outer medulla. ISOM - inner stripe outer medulla. IM - inner medulla. Jablonski scores are summarized in the bar graph (*p ≤ 0.001). (E) Renal fibrosis was analyzed by Masson’s trichrome staining (7 days after IRI). The bar graph compared the extent of renal fibrosis in the two groups (p=0.002). (F) KIM-1 expression immunohistochemistry (7 days after IRI). The bar graph summarized the results of KIM-1 staining in these mice (*p<0.002). (G) Renal apoptosis was analyzed by TUNEL staining (7 days after IRI). The upper panel showed representative microscopic images, the lower panel summarized quantification of apoptotic nuclei from the two groups of mice (*p<0.003). (H) Activation of caspase 3 and 9 in kidneys (7 days after IRI). Activated caspase 3 and 9 were analyzed by immunohistochemistry. The bar graph summarized the results of staining in these mice. *p < 0.001. (I) Glomerulosclerosis was analyzed by Periodic acid-Schiff (PAS) staining. The PAS results were graded as follows: 0 (no lesions), 1 (lesions in up to 25% of glomeruli), 2 (lesions in 25 to 50% of glomeruli) or 3 (lesions in > 50% of glomeruli). The representative images were shown in the upper panel. 50 glomeruli were scored for each mouse (15 mice in each group), the results were summarized in the graphs (lower panel, *p<0.003). (J) Survival analysis after IRI was compared in the two groups (TAM n=26, Corn oil n=24) (H). 76.9% of TAM-treated KMCAKT mice survived by day 7, as compared to 20.83% in control mice. Log rank test, p<0.0001.