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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Orthop Res. 2020 Oct 6;39(7):1452–1462. doi: 10.1002/jor.24865

FIGURE 5.

FIGURE 5

(A) Typical immunostaining image of FAPs under treatment BMP-7 and/or TSA. (B) Quantification of the percentage of perilipin A (+) FAPs in all treatment groups. Typical FAPs expressed UCP-1 were indicated with arrows in the TSA treatment group compared with FAPs without expression of UCP-1 in the DMSO group. (C) Quantification of the percentage of αSMA positive FAPs in all treatment groups. (D) RT-PCR data showed TSA significantly decreased adiponection and PPARγ gene expression, but increased UCP-1, collagen 1, and ACTA2 gene expression in FAPs in presence of BMP-7 (*p < .05). BMP-7, bone morphogenetic protein-7; DMSO, dimethyl sulfoxide; FAPs, fibro/adipogenic progenitors; RT-PCR, real-time polymerase chain reaction; TSA, Trichostatin A; UCP-1, uncoupling protein-1