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. 2021 Mar 10;11:546586. doi: 10.3389/fonc.2021.546586

Figure 2.

Figure 2

In vitro cytotoxicity of CP CAR-T cells against HCC cells. (A) Human CAR- T transduced cells or activated T cells were co-incubated with human MHCC97, HepG2 HCC cell lines, human normal liver epithelial cells LO2 and c-Met knockdown HepG2 cells (HepG2lo) for 12 h. The cell ratios of effector: target (E: T) was 10:1, 5:1, and 2:1, specific lysis was tested by LDH release assay. (B) At the cell ratios of E: T was 10:1, the specific cytotoxicity of different effector cells against various target cells. Data are representative of three independent experiments. Data are shown as mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001. (*CP CAR-T cells group compared with activated T cells, *marked with red color means CP CAR-T cells group compared with monovalent c-Met CAR-T cells group).