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. 2021 Mar 10;11:546586. doi: 10.3389/fonc.2021.546586

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Copyright © 2021 Jiang, Li, Guo, Wang, Jia, shi, Yang, Jiao, Wei, Feng, Tang and Ji

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CP CAR-T cells specifically inhibiting c-Met/PD-L1 expression in subcutaneous HCC tumors. (A) Schematic showing how to establish a bilateral subcutaneous tumor. (B) Tumor progression was assessed by bioluminescence assay on day 0 and 9 after CAR-T cells injection(n=5). (C) Quantification of tumor burden was depicted according to the bioluminescence signal. (D, E) Immunohistochemical (IHC) staining for anti-human c-Met were performed on tumor samples, representative images were taken with a microscope under 100x and 400x magnification. (F) Immunohistochemical (IHC) staining for anti-human Ki-67 were performed on tumor samples, representative images were taken with a microscope under 400x. Data are shown as mean ± SD. **p < 0.01.