FIGURE 3.

Thrombin induces tissue factor synthesis by a PAR1–PAR2 transactivation mechanism. (A,B) TF induction by the PAR1, PAR2 (2F), PAR3 and PAR4 activating peptides (the full sequences of these peptides are shown in the Methods section). Of the PAR-activating peptides only P2 (2F) induces TF synthesis. (C–E) Thrombin-induced TF synthesis is antagonized by the PAR1 antagonist Vorapaxar (4 μM) whereas 2F induced TF synthesis is not affected. Representative blots are shown (n ≥ 3). * and ** indicate significant fold changes of p < 0.05 and 0 < 0.1. An unpaired two-sided Student’s t-test was used.