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. 2021 Mar 10;11:638751. doi: 10.3389/fonc.2021.638751

Figure 3.

Figure 3

ZNF213 depletion inhibits ER alpha protein and ER alpha target genes in breast cancer cells (A, B) ZNF213 depletion effect on ER alpha protein level in MCF-7and T47D cells. Cells were transfected with siZNF213 or siControl. After 48 h, cells were treated with either ethanol or 10 nM estradiol for 6 h. ZNF213 and ER alpha protein levels were determined by Western blot analysis. Actin was used as internal control. (C, D) ZNF213 depletion affects ERE-luciferase activity in MCF-7and T47D cells. Cells were transfected with siZNF213 or siControl together with ERE luciferase reporter plasmid. Cells were treated with 10 nM estradiol or vehicle. Luciferase activity was measured 48 h after transfection. Shown are the results from three experiments. **P < 0.01 for luciferase activity comparison. (E, F) ZNF213 depletion decreases ER alpha target genes in MCF-7and T47D cells. Cells were transfected with siZNF213 or siControl. After 48 h, cells were treated with either ethanol or 10 nM estradiol for 6 h. Total RNA was prepared and the expression of the endogenous ER alpha target genes, PS2, GREB1, PKIB, and PDZK1 were determined by qPCR. Shown are the results from three experiments. *P < 0.05; **P < 0.01; ***P < 0.001 for target gene expression comparison.