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. 2021 Mar 10;11:638751. doi: 10.3389/fonc.2021.638751

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Copyright © 2021 Yang, Lv, Li, Mao, Niu, Wang, Zhuang and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ZNF213 could associate with ER alpha in breast cancer cells (A) Intracellular localization analysis of ZNF213 and ER alpha by immunofluorescence assay. MCF7 cells were cultured in normal medium before fixation. Intracellular localization of ER alpha (green) and ZNF213 (red) were shown. Nuclei (blue) were stained with 4’,6-diamidino-2-phenylindole (DAPI). (B) Co-IP assay reveals association between endogenous ZNF213 and ER alpha in MCF7 cells. MCF-7 cells were harvested with RIPA lysis buffer. CO-IP was performed using antibody as indicated. (C) ER alpha domain structure and deletion mutants used in the study (Full length, ΔAF1, ΔAF1+ΔDBD, ΔAF2, ΔAF2+ΔDBD). (D) ZNF213 full length and deletion mutants are used in the study (Full length, ΔZF domain, ΔLeR/SCAN domain, ΔZF+ΔKRAB-A domain). (E, F) AF1 domain is required for ER alpha to interact with ZNF213 (G) ZF domain is required for ZNF213 to interact with ER alpha.