Fig. 3. Renal interstitial cell-specific ablation of Sirt1 enhances fibrogenesis during UUO injury.

Male WT (Sirt1^flox/flox) and Sirt1 CKO (Sirt1^{flox/flox/TNCCreER}), at 8–10 weeks of age, were subjected to UUO operation. Mice were sacrificed on the 10th day after sham or UUO surgery. a Paraffin section from kidneys of sham or UUO surgery mice was analyzed by Masson-trichrome staining. Scale bar = 20 μm. b Quantitative analysis of Masson-trichrome staining. c Sirius red staining. Scale bar = 20 μm. d Quantitative analysis of Sirius red staining. e BUN levels. n = 6. Data are means ± SEM; ns: no significance. *P > 0.05, **P < 0.01, ***P < 0.001.