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. 2021 Mar 23;7:59. doi: 10.1038/s41420-021-00443-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a UUO injury increases HIF-2α protein levels in kidneys of WT and Sirt1 CKO mice. Protein levels were analyzed by western blot analysis and GAPDH was used as a loading control. b Quantitative analysis of western blot data as shown in a. c HIF-2α mRNA levels were not altered by UUO surgery. Gene expression was analyzed by qRT-PCR and 18S was used as a house-keeping gene. d–e HIF-2α in the renal tubule (d) and glomerulus (e) were analyzed by immunofluorescence. Scale bar = 20 μm. n = 6. Data are means ± SEM. ns: no significance. *P > 0.05, **P < 0.01.