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. 2021 Mar 23;11:6628. doi: 10.1038/s41598-021-85019-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative metabolic activity of the cells (resazurin assay) after 24 h incubation with extracts of Zn0.8 Mg. Three replicates 1–3 were used. The extract was added either to L929 or to U-2 OS cells. Sole extraction medium served as a control (unaffected cells which metabolic activity was taken as 100%). Numbers above the columns stand for the concentration of measured Zn in µM. The dashed line stands for the normative limit of 70% metabolic activity of the control. Error bars stand for the standard error deviation of six measurements. Significance codes according to p value: 0 ‘***’0.001 ‘**’0.01 ‘*’0.05 (two-sample t-test).