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. 2021 Mar 23;11:6679. doi: 10.1038/s41598-021-86146-w

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© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescent and immunoblotting detection of TLR4 pathway. (a) Immunofluorescence double staining of TLR4; (b) The double-blind counting of TLR4 positively-stained tubules (green-labeled), n = 12 per group; (c) Representative bands of Western blot on TLR4 and MyD88, n = 6 for each group. The results are expressed as the mean ± SD. ^*p < 0.05, ^**p < 0.01, v.s. sham; ^#p < 0.05, ^##p < 0.01, v.s. vehicle1; ^@@p < 0.01, ^nsp > 0.05.