Skip to main content
. 2021 Mar 23;12:1836. doi: 10.1038/s41467-021-22070-x

Fig. 6. Oncogene-induced epigenetic modifications increase the responder percentage.

Fig. 6

a Clonal MCF10A cell line containing TRE3G::BRAFV600E was immunostained for p65 after 24 h of culture in the presence or absence of Doxycycline (2 µg/ml) after Pam3CSK4 (1 µg/ml) stimulation for 30 min. Representative images are shown. Scale bar, 50 μm. b Monolayers treated with Pam3CSK4 (1 µg/ml) only, or increasing duration of dox, or ERKi (5 μM ulixertinib), prior to Pam3CSK4 treatment. Data represents three replicates. c TLR2 immunoblotting of BRAFV600E dox inducible cells as in panel b. HSC70 is loading control, relative amounts are reported with standard deviation representing two independent experiments. d TLR2 locus targeted nanopore methylation sequencing of genomic DNA isolated from human MCF10A cells with (Plus Dox, 96 h) or without (No Dox) BRAFV600E overexpression. e TRE3G::BRAFV600E monolayers were cultured with dox or inhibitors (HATi, A-485 (10 µM) and RSKi, BI-D1870 (10 µM)) and percent Pam3CSK4 (1 µg/ml) responders was calculated by NF-κB immunofluorescence. Error bars represent three technical replicates.