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. 2021 Mar 23;11:6617. doi: 10.1038/s41598-021-84278-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced population of Otp⁺ progenitors in the TPp of pink1^-/- zebrafish at 3 months and 24 months of age. (A) Quantification of the number of Otp⁺Th1^- progenitors and the number of Otp⁺Th1⁺ DA neurons in the TPp at 3 months shows a significant reduction in the number of Otp⁺Th1^- progenitors in the pink1^-/- zebrafish compared to pink1^+/+ zebrafish (t-test, p = 0.0292, n = 2 fish for each), but no significant difference in the number of Otp⁺Th1⁺ DA neurons. (B) Quantification of the number of Otp⁺Th1^- progenitors and the number of Otp⁺Th1⁺ DA neurons in the TPp at 24 months shows a significant reduction in the number of Otp⁺Th1^- progenitors in the pink1^-/- zebrafish compared to pink1^+/+ zebrafish (t-test, p = 0.0259, n = 4 fish for each), and in the number of Otp⁺Th1⁺ DA neurons (t-test, p = 0.0453, n = 4 fish for each).