Figure 10.

Fluorescence microscopy images of cryosectioned 3D JIMT-1/HDF co-cultures incubated in the absence or presence of TGF-β1. The JIMT-1/HDF 3D cultures were incubated in the absence (control) or presence of TGF-β1 (5 ng/ml). After 7 and 14 days of incubation, cultures were fixed in 3.7% formaldehyde, stained to visualize actin filaments (red), fibronectin (green), and cell nuclei (blue), followed by cryosectioning, and fluorescence microscopy. All images are representative of 3 independent experiments. Scale bar is 50 μm.