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. 2021 Mar 23;11:6655. doi: 10.1038/s41598-021-85742-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal images of actin filaments in HDF 3D mono-cultures incubated in the absence or presence of TGF-β1. HDF 3D co-cultures were incubated in the absence (A and C) or presence of 5 ng/ml TGF-β1 (B and D) for 7 and 14 days in 3D scaffolds, respectively. The cultures were fixed in 3.7% formaldehyde and stained to visualize actin filaments (red) and cell nuclei (blue). The images were taken in the centre of 3D cultures All images are representative of 3 independent experiments. Scale bar is 50 μm.