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. 2021 Mar 23;11:6655. doi: 10.1038/s41598-021-85742-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Focus stacking images obtained by confocal microscopy of fibronectin deposition and localization in 3D cultures of HDFs. Three confocal plane images were obtained with approximately 10 µm distance from each other in the 3D cultures. HDF 3D mono-cultures were incubated in the absence (control) or presence of 5 ng/ml TGF-β1 for 7 and 14 days. Cultures were fixed in 3.7% formaldehyde and labelled to visualize actin (red), fibronectin (green), and cell nuclei (blue). All images are representative of 3 independent experiments. Scale bar is 50 μm.