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. 2021 Mar 23;11:6655. doi: 10.1038/s41598-021-85742-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence microscopy images of cryosectioned 3D mono-cultures of HDFs incubated in the absence or presence of TGF-β1. The HDF 3D cultures were incubated in the absence (control) or presence of TGF-β1 (5 ng/ml). After 7 and 14 days of incubation, cultures were fixed in 3.7% formaldehyde, stained to visualize actin filaments (red), fibronectin (green), and cell nuclei (blue), followed by cryosectioning, and fluorescence microscopy. All images are representative of 3 independent experiments. Scale bar is 50 μm.