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. 2021 Mar 10;12:621744. doi: 10.3389/fimmu.2021.621744

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Copyright © 2021 Jeske, Yuan, Fu, Bunnell, Logan and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stem cell properties of hASCs during in vitro culture expansion. (A) Representative images of hASC morphology during culture expansion. Scale bar: 250 µm. (B) The population doubling time of hASCs at different passages. (C) mRNA levels of cell cycle markers of hASCs at different passages. mRNA levels of (D) stemness genes and genes for (E) osteogenic differentiation and (F) adipogenic differentiation of hASCs at different passages. (G) Colony-forming ability (CFU) and (H) β-gal activity of hASCs at P5 and P12. *indicates p < 0.05; **p < 0.01; ***p < 0.001.