Table 3.
Phenotypic characteristics of strain TW-1T that differentiates with phylogenetically related species of the genus Massilia.
| Characteristic | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
|---|---|---|---|---|---|---|---|
| Highest growth temperature (°C) | 42 | 37 | 37 | 37 | 42 | 40 | 42 |
| pH range | 4.5–10.5 | 5.5–9.0 | 5.5–8.5 | 5.0–9.0 | 6.0–8.5 | 5.5–9.0 | 5.0–9.0 |
| Highest NaCl tolerance (%, w/v) | 1 | 1 | 0.5 | 0.5 | 0.5 | 1 | 2 |
| Nitrate reduction | + | + | – | + | + | – | + |
| Urease activity | – | – | – | – | + | – | – |
| Hydrolysis of | |||||||
| Aesculin | + | + | – | – | + | + | + |
| Casein | + | – | – | – | – | – | + |
| Gelatin | w | – | + | – | – | + | – |
| Starch | + | – | + | – | + | + | + |
| Enzyme activity | |||||||
| Acid phosphatase | + | – | – | – | + | + | + |
| Alkaline phosphatase | + | + | + | + | + | + | w |
| Cystine arylamidase | + | + | + | – | + | + | – |
| Esterase (C4) | – | + | + | + | + | + | w |
| Esterase lipase (C8) | w | + | + | + | + | – | – |
| Leucine arylamidase | + | – | – | + | + | + | + |
| Lipase (C14) | w | – | – | – | – | – | – |
| N-acetyl-β-glucosaminidase | + | – | – | – | + | – | + |
| Valine arylamidase | + | – | – | + | + | + | + |
| α-galactosidase | + | + | + | + | + | – | + |
| α-glucosidase | + | + | + | – | + | – | + |
| β-galactosidase | + | + | – | – | + | + | w |
| β-glucuronidase | w | – | – | – | – | – | – |
| Assimilation from | |||||||
| 2-Ketogluconate | – | – | – | + | – | – | – |
| 3-Hydroxybenzoic acid | – | – | – | – | – | + | – |
| 3-Hydroxybutyric acid | + | – | – | – | – | + | w |
| 4-Hydroxybenzoic acid | + | – | – | – | – | + | – |
| Adipic acid | – | – | – | + | – | – | w |
| Capric acid | – | – | – | – | w | – | – |
| d-maltose | + | – | + | + | + | + | + |
| d-mannose | + | – | + | + | + | + | + |
| d-melibiose | + | – | – | – | – | – | – |
| d-saccharose (sucrose) | + | + | + | + | – | – | + |
| Glycogen | + | + | – | – | – | – | + |
| Lactic acid | + | – | – | – | – | – | w |
| l-alanine | + | – | – | – | – | + | – |
| l-arabinose | + | – | + | + | + | + | + |
| l-fucose | + | – | – | + | – | – | + |
| l-histidine | + | – | – | – | – | – | – |
| l-proline | w | – | + | – | – | + | – |
| l-rhamnose | + | – | – | – | + | + | + |
| Malic acid | – | – | + | + | – | + | + |
| N-acetyl-glucosamine | – | + | – | – | + | + | – |
| Phenylacetic acid | – | – | – | + | – | – | + |
| Potassium gluconate | – | – | + | + | + | + | w |
| Salicin | – | – | – | – | – | + | – |
| Sodium acetate | – | – | – | – | – | + | – |
| Trisodium citrate | – | – | – | + | w | – | w |
| DNA G + C content (mol%) | 66.3 | 69.4* | 64.7 | 66.2 | 63.2† | 66.3 | 66.7‡ |
Strains: 1, TW-1T; 2, M. pinisoli KACC 18748T; 3, M. putida KACC 21418T; 4, M. phosphatilytica KACC 21417T; 5, M. arvi KACC 21416T; 6, M. niastensis KACC 12599T; 7, M. kyonggiensis KACC 17471T. All data were obtained from this study except otherwise indicated. + , positive; w, weak; –, negative.