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. 2021 Mar 23;11:6608. doi: 10.1038/s41598-021-85569-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative cardiac anomaly in postnatal day 1 Nkx2-5^+/R52G mouse and control wild-type littermate. Control Nkx2-5^+/+ (left) vs. Nkx2-5^+/R52G (right): (A) dissected hearts, (B) heart tissue sections and corresponding micro-computed tomography images. Excessive trabeculation in Nkx2-5^+/R52G is indicated by arrows. LA left atrium, LV left ventricle, PT pulmonary trunk, RA right atrium, RV right ventricle, VSD ventricular septal defect.