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. 2021 Mar 23;11:6608. doi: 10.1038/s41598-021-85569-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design of gestational hyperoxia (40% O₂ saturation) and representative images of postnatal day 1 Nkx2-5 mutant hearts using micro-computed tomography (CT). (A) Timeline of experiment. (B) Representative 2D images demonstrating perimembranous and muscular VSD regarding their location (arrows). (C) Representative 2D and 3D micro-CT images showing ventricular trabeculation (arrowheads). (D) Quantification of area size of total ventricle, inter-ventricular septum, trabecular and compact layers. Data are expressed as mean ± S.D.; individual data are represented as circles. The Shapiro–Wilk normality test confirmed normal distribution of variables. *P ≤ 0.05. VSD ventricular septal defect. ImageJ 1.52d (http://imagej.nih.gov/ij) was used for analysis.