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. 2021 Mar 23;11:6608. doi: 10.1038/s41598-021-85569-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of Nkx2-5 protein in E12.5 Nkx2-5^+/R52G embryonic hearts with or without hyperoxia. (A) Representative Western blotting demonstrating Nkx2-5 and GAPDH proteins (see Supplemental Fig. S1). (B) Quantitative data for Nkx2-5 protein expression relative to GAPDH. (C) Real-time reverse transcriptase PCR of Nkx2-5 mRNA relative to ß-actin. The value in the Nkx2-5 mutant-normoxia was defined as 1. The Mann–Whitney U-test was used for analysis. Data are expressed as mean ± S.D.; individual data are represented as circles. *P ≤ 0.05. ImageJ 1.52d (http://imagej.nih.gov/ij) was used for analysis.