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. 2021 Mar 23;11:6670. doi: 10.1038/s41598-021-85678-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Areas of the inner and outer region of the virtual open field. (B) Binning used to calculate movement parameters based on line crossings. The arrow in the top left corner illustrates a line crossing counted as thigmotaxis. The arrow in the center region illustrates a line crossing counted as center ambulation. The arrow at the bottom illustrates a line crossing counted as center entry. (C) Starting positions. (D) Rating positions.