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. 2021 Mar 23;11:6615. doi: 10.1038/s41598-021-85296-1

Figure 6.

Figure 6

(A) Diagram of the acoustic treatment tank. The tank rested on top of two granite plaques separated by anti-vibratory rubber pads. Inside, a custom-made net cylinder containing zebrafish egg/larvae was suspended 7 cm above an underwater speaker (UW30, Lubel Labs, Ohio, USA) that rested on top of a polyurethane sponge. (B) Oscillogram of sound files used for playbacks. Control- silent conditions, CN- continuous noise at either 130 (CN130) or 150 dB re 1 μPa (CN150), IN- intermittent regime with random short noise segments (IN1): 5–12 s duration spaced by silent intervals of 1–120 s (total noise exposure of c. 15%); medium noise segments (IN2): 30–60 s interspaced by 1–10 min silence (similar noise exposure to IN1); and long noise segments (IN3) of 15 min separated by 15 min silent periods (about 50% overall noise). Illustration was created using Adobe Illustrator CC 2020 version 24.0.2 for Windows, Adobe Inc., CA, USA and Blender version 2.80 for Windows, Blender Foundation, AMS, EU.