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. 2021 Mar 23;11:6665. doi: 10.1038/s41598-021-85897-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Alpha-diversity analysis with Pielou’s J’, a metric of evenness, against various cytokines and chemokines localized to the intestinal region using (A) Pearson’s correlations with jejunum complement component 5a concentration (C5a, ng/ml) against number of distinct features, and (B) Kruskal–Wallis rank-sum tests with post hoc pairwise Mann–Whitney U tests with jejunum interleukin-6 concentration (IL-6, pg/ml) and (C) colon C5a concentration (ng/ml) against Pielou’s J’. The bottoms and tops of boxes indicate the first and third quartiles, respectively; whiskers indicate the 1.5 interquartile range beyond the upper and lower quartiles. Values outside the whiskers are indicated by gray circles; numbers in each box indicate sample sizes. Statistical significance in each categorical variable was evaluated using Kruskal–Wallis rank-sum tests with post hoc pairwise Mann–Whitney U tests at p < 0.05. *p < 0.05. C5a complement component 5a, IL-6 interleukin 6.