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. 2021 Mar 10;11:623276. doi: 10.3389/fimmu.2020.623276

Figure 6.

Figure 6

Contribution of CD8 and CD4 T cell subsets to pre- and posttransplant donor-specific alloreactivity assessed by T-cell receptor dependent activation-induced cell markers (AIM) by flow cytometry analysis. (A) Comparison of % of CD8+ AIM + T cells (CD69+CD137+) and AIM+ (OX40+CD137+) CD4+ T cells after allogenic (donor-specific stimulation) in DST− or DST+ patients. CD8+ AIM+: median 0.17% (0.05–0.42) vs 0.81% (0.48–2.09), p=0.041; CD4+AIM+: median 0.08% (0.008–0.19) vs 0.40% (0.2–0.56), p=0.029 in non alloreactive versus alloreactive patients, respectively. (B) CD8+/CD4+ AIM+ T-cell ratio in preDST+ and dnDST+ samples, respectively. Median 2.77 (0.6–6.3) in preDST+ vs. 1.13 (−2.3–1.79) in dnDST+, p=0.13. AIM, T-cell receptor dependent activation-induced cell markers; DST, donor specific T-cell alloreactivity; preDST, pretransplant donor specific T-cell alloreactivity; dnDST, de novo donor specific T-cell alloreactivity.